Identification of Human Polymorphisms in the Phenylthio- carbamide (PTC) Bitter Taste Receptor Gene and Protein
نویسنده
چکیده
This paper introduces a lab project developed for the Summer Teachers’ Workshop in Genomics at Amherst College, and is easily tailored to the weekly format of undergraduate laboratory courses in genetics, genomics, molecular biology, or evolution. The project examines single nucleotide polymorphisms associated with human taste sensitivity to the bitter compound phenylthiocarbamide (PTC). Human cheek cell DNA is amplified and sequenced using PTC-specific primers, and sequence variations in the PTC gene are correlated with taste sensitivity to PTC strips. A ‘dry lab’ version of the activity can also be done using pre-obtained DNA sequences from individuals with known PTC genotypes.
منابع مشابه
The Molecular Basis of Individual Differences in Phenylthiocarbamide and Propylthiouracil Bitterness Perception
Individual differences in perception are ubiquitous within the chemical senses: taste, smell, and chemical somesthesis . A hypothesis of this fact states that polymorphisms in human sensory receptor genes could alter perception by coding for functionally distinct receptor types . We have previously reported evidence that sequence variants in a presumptive bitter receptor gene (hTAS2R38) correla...
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